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| Figure 3: Electropherograms of 1.00 x 10-5 M HSA labeled on-column with different concentrations of bis-SQHN-4d present in the 25.0 mM phosphate buffer (pH 11.3). Dye concentrations as follow: (a) 1.00 x 10-6 M ; (b) 1.00 x 10-5 M; (c) 5.00 x 10-6 M. Electropherograms offset for clarity. Other separation conditions: 75 μm ID x 75 cm total length x 62.5 cm effective length capillary; capillary and sample temperatures held constant at 25°C; pressure injection at 50 millibar for 5 s; 20 kV separation voltage. |
