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| Figure 6: Electropherograms of a mixture of proteins with different pI’s labeled on-column with 5.00 x 10-6 M bis-SQHN-4d present in the 25.0 mM phosphate separation buffer (pH 11.3). Separation voltage: 20.0 kV; capillary and sample temperatures: 15°C; pressure injection: 50 millibar for 5 s; and capillary dimensions: (a) 75 μm ID x 75 cm total length x 62.5 cm effective length capillary, (b) 50 μm ID x 75 cm total length x 62.5 cm effective length capillary. Peak identities: I, 1.00 x 10-5 M (a) or 6.70 x 10-5 M (b) myoglobin (pI=7.16); II, 5.00 x 10-6 M (a) or 3.30 x 10-5 M (b) transferrin (pI=5.9); III, 5.00 x 10-6 M (a) or 4.00 x 10-6 M (b) HSA (pI=4.8). |
