Solvent or Buffer 7.00 x 10-6 M bis-SQHN-4d   no added protein with 1.00 x 10-6 M BSA     λabs,max (nm)   λemis,max (nm)   λabs, max (nm)   λemis, max (nm) methanol 414 550 --- --- citric acid (25.0 mM, pH 3.1) 421 470 421 470 Tris-HCl (25.0 mM, pH 7.4) 418 470 418 470 boric acid (25.0 mM, pH 9.1) 424 470 424 470 Na2HPO4 (25.0 mM, pH 11.3) 420 475 423 620   7.00 x 10-6 M (abs) or 7.00 x 10-7 M (emis) SQHN-3c   no added protein with 7.00 x 10-6 M BSA   λabs,max (nm)   λemis,max (nm) λabs, max (nm) λemis, max (nm) methanol 640 650 --- --- citric acid (25.0 mM, pH 3.1) 643 656 650 659 Tris-HCl (25.0 mM, pH 7.4) 634 655 643 655 boric acid (25.0 mM, pH 9.1) 636 654 647 652 Na2HPO4 (25.0 mM, pH 11.3) 634 651 637 651

Table 1: Absorbance and fluorescence properties for bis-SQHN-4d and SQHN-3c dyes in various solvent systems.

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