Dye Labeling Mode Linear Regression
[protein concentration (M) vs. peak height]
Correlation
Coefficient
Limit of
Detection (M)
  bis-SQHN-4d     on-column y =1.00 x 10-9x + 117 0.9998 3.42 x 10-8
pre-column y=2.93x + 0.0018 0.9996 8.70 x 10-7
SQHN-3c on-column y =256000x – 118000  0.9997  0.216 x 10-6
 
Table 2: LOD for pre-column and on-column labeling of HSA with bis-SQHN-4d and for on-column labeling of BSA with SQHN-3c, analyzed by CE-LIF.