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| Figure 5: Effect of endocytosis inhibitors on transfection efficiency. (A) Panc 1 cells were pretreated with chlorpromazine (100 μM), excess HSA (225 μg), filipin complexes (5 μg/ml), or cytochalasin B (125 μM) for 30 min prior to transfection with the standard formulation. The transfection efficiency of the cells transfected with the standard formulation was set as 100%. Mean ± S.E. were obtained from three separate experiments carried out in triplicate. Effect of endocytosis inhibitors on uptake of fluorescein-labeled material. (B) Fluorescence micrographs of Panc 1 cells treated without and with chlorpromazine prior to treatment with 10 μg/ml Texas Red-conjugated transferrin; filipin complexes prior to treatment with 2.5 μg/ml Alexa Fluor 488-cholera toxin subunit B; and cytochalasin B prior to treatment with 1 unit of Alexa Fluor 488-Phalloidin to identify F-actin. |
