a. The mice were assigned randomly into four groups (n=11-13 animals in each group): control, MSG (MSG+NSCs) MSG+FNSCL.
b. The mice were exposed to MSG (2.0 g/kg per day, ig) for 10 days.
c,d. On day 1 and day 10 after the 10-day MSG exposure (2.0 g/kg per day, ig), the animals in MSG+NSCs group received intracerebroventricular transplantation of 10μl of NSC suspension, approximately 1.0×105 cells, and the animals in MSG+FNSCL group received intracerebroventricular injection of 10 μl of cell-free filtrate of lysates of 1.0×105 NSCs, respectively.
e. The control mice without MSG exposure and the mice in MSG group were intracerebroventricularly administered with 10 l of DMEM instead of NCSc or FNSCL.
f. Y-maze test for 6 consecutive days started on day 12 after the last NSC transplantation or injection of FNSCL, and was repeated twice on day 27 and day 47.
g. The histopathology changes of the animal brains were examined on day 1 after the completion of Y-maze test.
