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Figure 1: Glioma cell lines express message for survivin. Expression of mRNA encoding survivin was analyzed in two glioma cell lines by RT-PCR. The origin of the RNA is indicated at the top of each lane. The amount of the template cDNA was adjusted by the 30-cycle amplification with primers for β2m as a housekeeping gene, then the adjusted amount of cDNA template was used for a 30-cycle amplification with primers for survivin. The results shown are from one experiment that has been repeated at least three times.
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