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| Figure 2: Identification of the pSurvivin95-104-specific cytotoxic T lymphocytes by HLA-A2-peptide tetramer staining. The frequency of HLAA2- peptide tetramer-binding CD8+ T lymphocytes before and after expanding with W6/32 antibody-mediated pSurvivin95-104-specific aAPCs was shown. The following HLA-A2-peptide tetramer was used: HLA-A2-pSurvivin95-104 tetramer and the control tetramer HLA-A2-pHIV tetramer. A: The frequency of HLA-A2-pHIV tetramer-binding CD8+ T lymphocytes before stimulation was 0.57%; B: The frequency of HLA-A2-pSurvivin95-104 tetramer-binding CD8+ T lymphocytes before stimulation was 0.63%; C: The frequency of HLA-A2-pHIV tetramer-binding CD8+ T lymphocytes after stimulation was 0.82%; D: The frequency of HLA-A2-pSurvivin95-104 tetramer-binding CD8+ T lymphocytes after expansion was 95.1%. These results are representative of three experiments, and demonstrate that the co-culture of W6/32 antibody-mediated pSurvivin95-104-specific aAPCs and HLA-A2 positive PBLs can induce the pSurvivin95-104- specific CTLs. |
