| Immunolabeling showed robust expression of Cx43 (red label) at the intercalated
discs in sections of freshly cut adult rat heart slices (A). Nuclei were counter-stained in blue with DAPI in the fixated sections. After 1 day in culture (B) the intensity of Cx43 labeling was much reduced. The Cx43 labeling was further reduced after 3 days in culture (C) and was non-existent after 6 days (D). One representative picture from 4 slices (from 4 different hearts) at each time point is presented. Bar markers = 100 μm. |
