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| Figure 1: RT-PCR analysis of endogenous expression of humanins HNM, HN10d and HN10dV in LN18. The cells were lysed with TRIzol® Plus RNA Purification System (Invitrogen). Obtained total RNA was reversely transcribed into cDNA, then subjected to PCR with the primers corresponding to the humanins listed above. A. Amplification plot of HN M, 10d and 10dV in LN18 cells. B. Amplification plot of HN M, 10d and 10dV in the LN18 cells incubated with 25 mM β-amyloid (βA) for 18 hours prior to their harvest. Insert, fold difference in threshold values for HN M, 10d and 10dV in the Ab-treated cells compared to their controls. Expression rate of each probe was adjusted for the amplification efficiency of the housekeeping gene, GAPDH. |
