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| Figure 3: Humanins-mediated effect on mitochondrial calcium retention capacity (CRC). The mitochondria were isolated from the rat livers, suspended in glutamate/ malate-containing solution and incubated with a selected HN for 15 minutes prior to calcium titration. Calcium ions (CaCl2) were added in 10 μM doses until opening of calcium megachannel (MMC) and complete calcium release from the mitochondria. A) Example of calcium titration in the control mitochondria (ctrl1, ctrl2) and mitochondria incubated with HN G. B) The CRC were adjusted for protein content, and normalized to the control (mitochondria incubated in absence of humanin) from the respective experiments. CRC in the control measurements were at the level of 0.12 ± 0.06 μmol Ca2+ / mg protein. Each experiment was repeated 3-5 times. |
