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| Figure 4: Analysis of mitochondrial swelling upon incubation with humanins. The mitochondria were isolated from the rat livers, suspended in glutamate/malate-containing solution, and incubated with selected HN for 15 minutes prior calcium addition. Calcium ions were titrated in two doses, 0.5 mM and 1 mM, and decrease of the absorbance signal at 540 nm marked mitochondrial swelling due to opening of the calcium megachannel (MMC) was recorded. Positive control was Cyclosporine A (CsA), which represented a potent MMC blocker. |
