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| Figure 6: Effect of Oct-4 knock-down on the expression of markers for undifferentiated spermatogonia. RT-PCR of A(i) Plzf, B(i) Gfra-1, C(i) c-Ret, D(i) Bcl6b, E(i) Etv5 and Relative expression of A (ii) Plzf, B (ii) Gfra-1, C (ii) c-Ret, D (ii) Bcl6b, E (ii) Etv5 by Q-PCR in Oct-4 shRNA treated and untreated cells. Negative control did not contain RT enzyme in the reaction mixture. Values are the mean ± SD. n=3 different biological replicates performed on three different occasions (*p<0.05). |
