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| Figure 7: Effect of Oct-4 knock-down on the expression of markers for undifferentiated spermatogonia at the protein level. Western blot analysis of A (i) PLZF, B (i) GFRA-1, C (i) c-RET, D (i) BCL6B, E (i) ETV5 and densitometry analysis of A (ii) PLZF, B (ii) GFRA-1, C (ii) c-RET, D (ii) BCL6B, E (ii) ETV5 in Oct-4 shRNA treated and untreated cells. The replacement of primary antibody with PBS was used as a negative control. Values are the mean ± SD. n=3 different biological replicates performed on three different occasions (*p<0.05). |
