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| Figure 3: Changes in lipid species content during SVF differentiation. SVF cells were incubated for 48 hours in adipogenic MDI medium for initiation of differentiation (Ctrl (+) 48 h) and then without differentiation factors for 15 days (Ctrl (+) 15d). The negative control cells (Ctrl (-)) were incubated without differentiation factors. The results are presented as average lipid species concentration (nmol/mg protein) detected using mass spectrometry ESI-MS/MS (n=3). Significant differences in relation to the control (-) 48 h are indicated by asterisks, *p<0.05. |
