control (-) 48h control (+) 48h control (-) 15d control (+) 15d SCD1/SCD5         18:1/18:0 1.82 2.83 1.83 4.03 DNL         18:2/16:0 0.026 0.029 0.028 0.038 ELOVL3/ELOV6         18:0/16:0 0.31 0.25 0.35 0.21 22:0/20:0 1.53 3.30 2.01 3.24 18:1/16:1 5.88 7.81 7.04 6.92 22:1/20:1 3.81 4.40 3.83 5.50 ELOVL5         20:3/18:3 8.06 15.90 8.03 14.67 FADS1         20:4/20:3 5.56 3.25 8.15 1.65 FADS2         20:3/18:2 1.63 2.99 1.38 3.15 PLAG2G6         20:5+22:6/20:4 0.452 0.411 0.414 0.374 PC/FC 0.999 1.416 1.008 1.635

Table 2: Changes in enzyme activity during SVF cell differentiation. SVF cells were incubated for 48 hours in adipogenic MDI medium for initiation of differentiation (Ctrl (+) 48 h) and then without differentiation factors for 15 days (Ctrl (+) 15d). The negative control cells (Ctrl (-)) were incubated without differentiation factors. Results calculated as the ratio of product/substrate for enzymes associated with lipid/fatty acid metabolism, determined by ESI-MS/MS, (n=3).

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