Human monocyte derived DCs were pulsed for three days with indicated concentrations of S100A4 and co-cultured afterwards with autologous lymphocytes for 5 (±1) days. Thus treated lymphocytes were co-cultured with HLA-A2 mismatched allogeneic lymphocytes for further 5 (±1) days in a 1:1 ratio and proliferation was assessed by flow cytometry. Inhibited proliferation of allogeneic lymphocytes is shown in panel A demonstrating unchanged cell count, while the number of whole autologous lymphocytes and CD4+CD25+ autologous lymphocytes increased significantly at about 25% (B) and 50% (C), respectively. Asterisks indicate p ≤ 0.05.
