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| Figure 5: In vivo expression of germ cell specific marker genes in differentiated SSCs obtained from the testes of wild type and c-Kit-ORF-GFP transgene insert and 1.1 kb control-GFP insert introduced mice testis on post GCT day 56. Oct-4 (466 bp), c-Kit (403 bp), Rbm1 (209 bp), Pgk-2 (353 bp), Hils-1(218 bp), Gata-1 (242 bp), Pem-1-(566 bp), Lhr (215 bp) and Tnp2 (255 bp) genes were expressed in the testis of wild type and c-Kit-ORF-GFP transgene construct introduced SSCs. The expression of Oct-4, c-Kit, Rbm- 1, Pgk-2, Hils-1 and Tnp-2 genes were absent in the testes of control-GFP insert introduced mice, whereas the expression of Sertoli cells (Gata-1, Pem-1) and Leydig cells (Lhr) markers was observed. Lane: 1 (wild type mice testis), Lane: 2 (c-Kit-ORF-GFP insert introduced mice testis) Lane: 3 (control-GFP insert introduced mice testius). Cyclophilin-A (465 bp) was used as an internal loading control. Figures shown are the representative pictures from two different experiments performed on two different days. |
