| Gene |
Gene Accession No |
Primer Sequence (5’-3’) |
Prod
Size
(bp) |
| SSCs differentiation markers |
| c-Kit |
NM_001122733.1 |
F- ACCCACAGGTGTCCAATTATTC |
403 |
| R-TGGCGTTCATAATTGAAGTCAC |
| Rbm-1 |
NM_001270510.1 |
F- AACCGAAGTAACATATACTCA |
209 |
| R-ATCTGCTTTCTCCACGACCTC |
| Oct-4 |
NM_013633.3 |
F-AGCTGCTGAAGCAGAAGAGG |
466 |
| R-TGGGAAAGGTGTCCCTGTAG |
| Gfp |
M62653 |
F- AGGACGACGGCAACTACAAG |
312 |
| R-CTGGGTGCTCAGGTAGTGGT |
| Sertoli cell specific genes |
|
| Gata-1 |
NM_008089.1 |
F-TGTGTGAACTGTGGAGCAACGGC |
242 |
| R-AAATGAAGGCCGCAGGCATTGCA |
| Pem-1 |
NM_008818.2 |
F-AAGAACAGCATGATGTGA; |
566 |
| R-TCAAAATCTCGGTGTCGCAAA |
| Haploid cell marker genes |
| Tnp2 |
NM_013694.4 |
F-CGGCCTCAAAGTCACACCAGT |
255 |
| R- AGTCCGTTTCCGCCTCCTGAC |
| Hils-1 |
NM_018792.1 |
F-TGGAGTATCTAGCACCTGGAGT |
218 |
| R-TCAGAATCACATACGACATGGT |
| Leydig cell marker gene |
|
| Lhr |
NM_013582.2 |
F- AATCCCATCACAAGCTTTCAG |
215 |
| R- TGCCTGTGTTACAGATGC |
|
| Spermatocyte marker |
|
| Pgk-2 |
NM_031190.2 |
F- GACAATGGAGCCAAGTCCGT |
353 |
| R- GCAGTGCCAAATGCATCGTT |
| House keeping genes |
|
| Cyclophilin-A |
NM_008907.1 |
F-AAGTTCCAAAGACAGCAGAAAACT |
465 |
| R-GAGCTACAGAAGGAATGGTTTGAT |
| Gapdh |
NM_008085.1 |
F-CCATTCATTGACCTCCACTACA |
199 |
| R-CGTTGCTGACAATCTTGAGAGA |
