Primer type Sequence Annealing Temperature Amplified product P53 Forward (wild ) 5’-CTTGTCCTGGGAGAGACC-3’ 58°C 207 bp P53 Forward (mutant ) 5’-CTTGTCCTGGGAGAGACT-3’ P53 Reverse (common) 5’-GAGCTGGTGTTGTTGGGC-3’

Table 5: Primer sequence for ASO-PCR used for p53 exon 8 codon 282 DNA-binding domains.

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