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| Figure 3: Regulatory effects of TSLP shRNA on the expression of TGF-β RI, pSmad2/3, and fibronectin in HFL-1 cells. (A) Cells were manipulated and transfected as above. The cells were subjected to stimulation of TGF-β1 (10 ng/ml) for 24 hours in serum-free medium. Whole-cell lysates were immunoblotted with anti-TGF-β RI, anti-pSmad2/3, and anti-fibronectin antibodies. (B) Densitometry analysis from Figure 3B shows the group examined by anti-fibronectin. P## <0.01 versus control. P**<0.01 versus the TGF-β1 control (i.e., the TGF-β1 treated group). The experiment was repeated twice and consistent results were obtained. |
