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| Figure 7: Collagen-rich areas of fibrosis are smaller in TSLP shRNA-inoculated mice in bleomycin modeling. Regardless of treatment with bleomycin or saline, mice pulmonary tissue samples were perfused, fixed, and embedded in paraffin. Sections were stained for collagen with Sirius red (red) and for noncollagenous protein with fast green FCF (green), and then photographed at a magnification of 10. Bleomycin-treated (B, F) animals intensely stained for collagen around large airways and blood vessels; however, both wild-type control (A, E) and TSLP shRNA transgenic control (C, G) animals had foci of fibrosis that stained for collagen. More importantly, pulmonary tissue from a bleomycin-treated mouse inoculated with TSLP shRNA (D, H) and sacrificed at 7 and 21 days shows significantly decreased fibrosis in collagen stain compared to the bleomycin-treated group. |
