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| Figure 1: Characterization of ERα-OE mice. (A-C): Representative Western blots demonstrate that A: ERα levels are significantly higher in the LV tissue of female and male ERα-OE mice compared to WT-mice, B: ERα protein is overexpressed only in the LV tissue, but not in soleus muscle, uterus, lung and kidney of ERα-OE mice. Uterus was used as positive control. C: The phosphorylation level of ERα at Serin-118 is significantly higher in the LV of both female and male ERα-OE mice compared to those in WT-mice. GAPDH was used as loading control protein for Western blot analysis and protein normalization. ERα/GAPDH and pERα/GAPDH ratios are indicated below each Western blot (A&C). Each bar represents the mean ± SEM (n=7-10 mice per each group). § for the genotype effect (ERα-OE vs. WT, p<0.001). D: Immunofluorescence analysis followed by confocal laser microscopy of 3μm paraffin sections revealed higher expression and more intense nuclear localization of transgenic ERα (green) in the cardiomyocytes of ERα-OE mice. Nuclei are shown in blue (Dapi). 20x magnification, scale bar 250μm. |
