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| Figure 4: Western blot analysis of TATκ-Oct-3/4 and TATκ-KLF4 transfected 293T cells. Intracellular expression (cell lysates) and secretion of TATκ fusion proteins in the culture medium, 3 days after transfection of 293T cells with either TATκ-Oct-3/4 or pTATκ-KLF4 plasmid DNA. (a) TATκ-Oct-3/4 protein (~47 KDa), and (b) TATκ- KLF4 protein (~60 KDa). Cell lysates and concentrated culture medium from untransfected 293T cells were used as negative controls. Commercially available mouse embryonal carcinoma (F9) and mouse testis extract cell lysates were used as positive controls. An anti-α-Tubulin antibody was used to detect α-Tubulin (~ 55 KDa), which was used as a loading control for each sample. Similar results were obtained in three independent experiments. |
