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| Figure 7: Validation of TATκ-OCT-3/4 secreted proteins in concentrated culture medium by Western blot analysis. The ‘factor producing cells’ were grown till 80-90% confluent in complete growth media (supplemented with 10% heatinactivated FBS). The media was replaced with 10mL of serum free-media and incubated for a further 24 hours. The media containing TATκ-OCT-3/4 protein was concentrated using Vivaspin column (20 x concentrations). The presence of TATκ-Oct3/4 protein was detected using rabbit polyclonal antibody. The culture medium from wild-type 293T cells was used as a negative control, while a commercially obtained mouse embryonal carcinoma (F9) cell lysate was used as positive control. |
