Figure 1: A) IFN-g reduces the sensitivity of MSC to IL2-activated NK killing. MSC cultured in the presence or absences of IFN-g (20ng/ml) for 48h were used as target in a non-radioactive cytotoxicity assay (Promega) with NK effector cells at different ratio NK/MSC. B) IFN-g reduces the sensitivity of MSC to IL2-activated NK killing. CD107a degranulation by NK cells against IFN-primed MSC. NK cells were cultured with MSC (ratio NK/MSC, 5:1) for 4h in presence of anti-CD107a antibody and Monensin (2 mM). K562 cell line was used as positive control. ***=p<0.001, **=p<0.05 C and D: Degranulation ability of NK cells (CD107a assay) was remarkably decreased against IFN-γ-primed MSC as demonstrated by the decreased percentage of CD56+/CD107a+ cells when BM/FL/ES-MSC were primed with IFN-γ

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