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Figure 4a: MSC impair NK cell lytic functions. MSC cultured in the presence or absences of IFN-γ (20ng/ml) for 48h were used as target in a non-radioactive cytotoxicity assay (Promega) with NK effector cells at different ratio NK/MSC. K562 cell line was used as positive control. One representative out of 5 independent experiments is presented. Data are shown as box plots. Each box represents the 25th to 75th percentiles. Lines outside the boxes represent the minimum and maximum values. Lines inside the boxes represent the mean. |