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| Figure 2: Cells morphology and cellular composition after treatment with 1% GNS, NT 0.1 mg/ml, GNS combined with NT and in control samples. Images taken using inverted fluorescent microscope were shown in the figures A-F. Figure A-D presents cells after DAPI staining, figures E-H after atto-488-phalloidin staining and figures I-L after MitoRed staining. Microphotographs obtained using scanning electron microscopes are presented in the figures M-P. Results of quantitative microvesicles’ analysis performed with Image J are presented in the figures Q-T. For fluorescent microscope scale bars are 200 μm, for SEM scale bars are 10 μm. Multipolar cells were marked with white arrows, cells characterized by bipolar morphology were indicated with black arrows, while cells aggregates were shown using white arrowheads and proper developed cytoskeleton by white asterisks. Mitochondria were marked using black arrowheads, while apoptotic bodies were pointed out using red asterisks. Microvesicles were indicated with red arrows |
