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| Figure 4: (A) Calcein-positive BM-MNCs and calcein-positive rodshaped cardiomyocytes after 24 h coculture. Zeiss Axiovert 40C/CFL epifluorescence microscope, objective magnification 40×. (B) Control positive staining of alpha-actininsarcomeric structure inside adult cardiomyocyte (left) and neonatal cardiomyocyte(right). Stained by anti-alpha-actinin antibody and secondary anti-mouse IgG1–FITC antibody. Blue points are nuclei stained with DAPI. Generated by Zeiss LSM700 confocal microscope, objective magnification 63×. Scale bars represent 40 μm. |
