| Gene Name |
Sequences 3à5 |
Tm |
| Oct3/4 |
Forward: 5' -CTGAGGGCCAGGCAGGAGCACGAG-3'
Reverse: 5' -CTGTAGGGAGGGCTTCGGGCACTT-3' |
59 |
| Nanog: |
Forward: 5' -TTCCTTCCTCCATGGATCTG-3'
Reverse: 5' -TCTGCTGGAGGCTGAGGTAT-3' |
61 |
| Sca-1: |
Forward: 5' -ACAGCCTGAGCAAGACCTGT-3'
Reverse: 5' -ATCATCCACCCCAGAAGTGA-3' |
60 |
| Sox2: |
Forward: 5' -GGTTACCTCTTCCTCCCACTCCAG-3'
Reverse: 5' -TCACATGTGCGACAGGGGCAG-3' |
60 |
| GAPDH: |
Forward: 5' -CCG CAT CTT CTT GTG CAG TG-3'
Reverse: 5' -CTG TGG TCA TGA GCC CTT CC-3' |
59 |
| Cdx4: |
Forward: 5' -CGTGTGGTCTACACAGATCATCAA-3’
Reverse: 5′ -GGCTCTGCGATTCTGAAACC-3’ |
62 |
| Rex1: |
Forward: 5' -ACGAGTGGCAGTTTCTTCTTGGGA-3'
Reverse: 5' -TATGACTCACTTCCAGGGGGCACT-3' |
61 |
| Osteopontin |
Forward: 5'- TTTTCTGGATCCTCCATTGC-3'
Reverse: 5'-CAAAAGCCATATGCTGCTCA-3' |
60 |
| Osteonectin |
Forward: 5'- GATGGTGCAGAGGAAACCGA-3'
Reverse: 5' TTTGCAAGGCCCGATGTAGT-3' |
61 |
| Osteocalsin |
Forward: 5'- GACTGTGACGAGTTGGCTGA-3'
Reverse: 5'-CTGGAGAGGAGCAGAACTGG-3' |
59 |
| Alkaline phosphates |
Forward: 5'-CCCAAAGGCTTCTTCTTG-3'
Reverse: 5'- CTGGTAGTTGTTGTG AGCAT-3' |
61 |
