| Species |
Gene Target |
Primer name |
Nucleotide sequence 5`-3` |
Working concentration (µM) |
| N. meningitidis |
ctrA |
F753 |
TGTGTTCCGCTATACGCCATT |
7.5 |
|
R846 |
GCCATATTCACACGATATACC AACCTTGAGCAA”T”CCATTTATCCTGACGTTCT |
22.5 |
| Pb820i |
5`FAM;3`SpC6;”T”BHQ1 |
2.5 |
| H. influenzae |
Hpd |
HPDF729 |
AGATTGGAAAGAAACACAAGAAAAAGA |
7.5 |
| HPDR819 |
CACCATCGGCATATTTAACCACT |
2.5 |
| PBR762i |
AAACATCCAATCG”T”AATTATAGTTTACCCAATAACCC
5`HEX;3`SpC6;BHQ1 |
5 |
| S. pneumoniae |
lytA |
F373 |
ACGCAATCTAGCAGATGAAGCA |
5 |
| R424 |
TCGTGCGTTTTAATTCCAGCT
TGCCGAAAACGC”T”TGATACAGGGAG |
5 |
| Pb400i |
5`Cy5;3`SpC6;”T”BHQ2 |
5 |
