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Figure 2: Effect of single and combination treatment on EGFR-related protein levels in SQ20B, SCC25 and SCC61 cells. The effects of each single treatment (erlotinib, celecoxib and ionizing radiation) and the combinations on p-EGFR/EGFR, p-AKT/AKT, p-ERK/ERK, p-STAT3/STAT3 and actin were assessed by immunoblotting. We assessed protein levels at 20 minutes and 72 hours after treatment to observe a time course of protein activation. Differences in protein levels were assessed by densitometry and are shown in Supplemental figures.
a. SQ20B
b. SCC25
c. SCC61
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