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Figure 4: Effect of BTP2 on [Ca2+]i in Jurkat cells stimulated by OKT-3 and CD28.6. After Jurkat cells had been cultured in pH 7.6 and 6.3 media for 24 h, they were treated with BTP2 (10 μg/ml) for 10 min and then stimulated with OKT-3 (0.2 μg/ml) and CD28.6 (5 μg/ml) for 5 min. [Ca2+]i was measured as described in Materials and Methods. The means and standard deviations of three measurements obtained from cells cultured independently are represented. p values of statistical analysis are represented. |