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| Figure 6: Phosphorylation of ERK and p38 upon the addition of OKT-3. Jurkat and P116 (ZAP-70-) cells cultured in pH 7.6 and 6.3 media for 24 h were treated with or without OKT-3 (0.2 μg/ml) for 5 min (A) or 30 min (B). Whole cell extracts were analyzed using anti-p-ERK1/2 mAb, anti-ERK1 mAb, anti-p-p38 mAb, and anti-p38 mAb as described in the legend of Figure 1. |
