Primer Pair

Primer Sequence
(F=forward, R=reverse)

Cycle number

Annealing (°C)

ChAT (total)

(F) 5’-caaggccacttagcctcctcggc-3’
(R) 5’-cttcaggagcagaagcttctcag-3’

30

62

M-ChAT specific

(F) 5’-ggagggaggaggtacaagaggaag-3’
(R) 5’-cggtttggacagtcagtgggaatg-3’

37

57.5

nNOS

(F) 5’-cccacgtggtcctcattctg-3’
(R) 5’-cattctttgtgggggactgtttt-3’

35

60

iNOS

(F) 5’-ccgcttcgatgtgctgcctct-3’
(R) 5’-cccatcctcctgcccacttcctc-3’

37

60

eNOS

(F) 5’-ggccccagcatccctactcc-3’
(R) 5’-gaacaccagctcgctctccctaag-3’

37

62.5

TrkA

(F) 5’-aaccagcccacccacgtcaacaac-3’
(R) 5’-cgccctcccccagctcccactt-3’

36

65

β-actin

(F) 5’-atgtttgagaccttccaacacccca-3’
(R) 5’-gaagagtgcctccagggcagcgga-3’

25

65