Figure 2: Western blot of PKCδVIII and Bcl2 expression in human neuronal (SH-SY5Y) and monocytic cell lines (THP-1) incubated with G-CSF for 24h (100 ng/ml or 200 ng/ml). Both PKCδVIII and Bcl2 expression are increased in the neuronal cells (100 ng/ml; 5.3 nM), and in the monocytic cells (200 ng/ml; 10.6 nM). The above graphs (B) and (D) show PKCδVIII or Bcl2 percent densitometric units normalized to GAPDH, and represent three separate experiments. The results were analyzed with two-tailed Student’s t-test. *** p<0.0001.
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