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Figure 2: Increase in membrane permeability of rat astrocytes treated with H2O2. Change of cytosolic protein level in rat astrocytes was analyzed after the pretreatment with 100 μM H2O2 for 10 min and then with exogenous trypsin (A). Inflow of Rhodamin-phalloidin into rat astrocytes was observed 0 or 1 h after the pretreatment with (0 h and 1 h) or without (Control) 100 μM H2O2 for 10 min and washing (B). |