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| Figure 4: Effect of H2O2 on syntheses of lipids such as cholesterol or sphingomyelin in rat astrocytes. After treatment with 100 μM H2O2 for 10 min and washing, rat astrocytes were incubated for 0, 3, 6, or 24 h in 0.1% BSA/F-10 medium and then incubated with 14C-acetate in a fresh 0.02% BSA/F-10 for 2 h for lipid synthesis. Lipids were extracted from whole cells and analyzed by thin layer chromatography for cholesterol (Cho) and Sphingo Myelin (SM). The radio activities of cholesterol and sphingomyelin were determined using a liquid scintillation counter. The data points represent the means ± SD, **, P<0.01 significantly different from the value of cells treated without H2O2. |
