Figure 4 | In-vitro cleavage of Hsp70.1 being involved..

Figure 4: In-vitro cleavage of Hsp70.1 being involved in various brain tissues from the non-ischemic monkey by activated μ-calpain.
A: 0.5 U μ-calpain being activated by 1.0 mM of CaCl₂ failed to cleave Hsp70.1 in the CA1 tissue sufficiently (dot red circle), but after carbonylation by 1.0 mM H₂O₂, thecleavage of Hsp70.1 occurred more drastically not only in CA1 (red circle) but also in other brain regions. HNE showed similar facilitation effects upon calpain-mediated Hsp70.1 cleavage (data not shown here). CA1: cornu Ammonis 1, DG: dentate gyrus.
B: Blockage of calpain-mediated cleavage of carbonylated-Hsp70.1 in CA1 by a specific calpain inhibitor ALLN. ALLN dose-dependently blocked cleavage of Hsp70.1 being carbonylated by 500 μM HNE. Similarly, ALLN dose-dependently blocked cleavage of Hsp70.1 being involved in other brain regions.

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