Figure 1: Human neuronal-glial (HNG) cells in primary culture – (A , Left panel) two weeks in culture; approximately 50-50 neurons and astroglia; neuronal cells are stained with neuron-specific β-tubulin (red; λmax=690 nm), glial cells are stained with glial-specific glial fibrillary acidic protein (GFAP; green; λmax=525 nm), and nuclei are stained with Hoechst 33258 (blue; λmax=470 nm); magnification 20x; (B, Right panel) co-incubation with H2DCFDA indicates ROS generation; treatment after 3 hrs with Al+Fe (50 nM each, as sulfates) shown; green fluorescence (emission λmax=530 nm).
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