Figure 2: Trypan blue stained primitive cells from adult equine blood after ingestion of AFA, original magnification, 200x. Note increased number of spherical-shaped stained and unstained structures, compare to Figure 1. Pluripotent stem cells (white arrows), negatively stained for Trypan blue. Cells have machinery to pump out Trypan blue dye and therefore appear as white “glowing” balls. Totipotent stem cells (black arrows), positively stained for Trypan blue. Cells lack machinery to pump out Trypan blue dye and therefore appear as dark spherical balls.

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