Characteristics Progenitor Cells Ectodermal, Mesodermal, &Endoderm Stem Cells Pluripotent Stem Cells Totipotent Stem Cells Size Variable 10-20 μm 6-8 μm <2 μm Trypan Blue Staining Negative Negative Negative Positive Cell Surface Markers Variable Thy-1+, CD90+, Variable dependent on Germ Layer Linege SSEA+, CD10+, CD1a-, CD2-, CD3-, CD4-, CD5-, CD7-, CD8-, CD9-, CD11b-, CD11c-, CD14-, CD15-, CD16-, CD18-, CD19-, CD20-, CD22-, CD23-, CD24-, CD25-, CD31-, CD33-, CD34-, CD36-, CD38-, CD41-, CD45-, CD49d-, CD55-, CD57-, CD59-, CD61-, CD62e-, CD65-, CD66e-, CD68-, CD69-, CD71-, CD79-, CD83-, CD90-, CD105-, CD117-, CD123-, CD135-, CD166-, Glycophorin-A-, MHC-I-, HLA-DRII-, FMC-7-, Annexin-V-, LIN- CEA-CAM+, CD66e+, CD1a-, CD2-, CD3-, CD4-, CD5-, CD7-, CD8-, CD9-, CD10-, CD11b-, CD11c-, CD14-, CD15-, CD16-, CD18-, CD19-, CD20-, CD22-, CD23-, CD24-, CD25-, CD31-, CD33-, CD34-, CD36-, CD38-, CD41-, CD45-, CD49d-, CD55-, CD57-, CD59-, CD61-, CD62e-, CD65-, CD68-, CD69-, CD71-, CD79-, CD83-, CD90-, CD105-, CD117-, CD123-, CD135-, CD166-, Glycophorin-A-, MHC-I-, HLA-DRII-, FMC-7-, Annexin-V-, LIN- Cryopreservation Temperature -196°C -70°C -80°C -80°C Log Phase Doubling Time Days to Weeks 18-24 hours 12-14 hours 12-14 hours Growth at Confluence Contact Inhibited Contact Inhibited Multiple Layers Multiple Layers Replicative Potential Hayflick’s Limit Extensive Extensive Extensive Differentiation Potential Cell Specific Ectodermal, Mesodermal, & Endodermal Lineage Cells All somatic cells, will not form gametes All somatic cells, will form spermatogonia

Table 1: Characteristics of animal and human precursor cells.

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