Figure 2: Expression of leptin protein in the tibiae of mice after OVX or sham operation. A. Immunohistochemical localization and expression of leptin in the tibiae of OVX and sham-operated mice, counterstained with hematoxylin. DAB and hematoxylin staining. Scale bars = 50 μm. B. Quantitative analysis of leptin protein in femora at 1 and 3 weeks after OVX or sham operation. Values are mean ± SE from two independent experiments performed in quadruplicate. *p<0.05.
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