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| Figure 4: Effect of leptin on bone marrow stromal cells. A. RT-PCR analysis for two variant leptin receptors, Ob-Ra and Ob-Rb. MC3T3-E1 cells were used as the positive control for leptin receptor expression. B. Western blot analysis for two variant leptin receptors, Ob-Ra and Ob-Rb. MC3T3-E1 cells were used as the positive control for leptin receptor expression. C. Effect of leptin on proliferation of bone marrow stromal cells. Values are mean ± SE from two independent experiments performed in triplicate. D-F. Expression of mRNA for osteoblastic differentiation markers, Col I, BSP and OC. Values are mean ± SE from two independent experiments performed in triplicate. **P<0.01. |
