Primers (GeneBank accession no.) Sequence Size (bp)
Type I collagen (NM_007742.3) F: 5’-CACTGCCCTCCTGACGCAT-3’ R: 5’-CACACAGCCGTGCCATTG-3’ 137
Bone sialoprotein (NM_008318.3) F: 5’-CCAGGACTGCCGAAAGGAAG-3’ R: 5’-CCCCGTTTTCTTCAGAATCCTCTG -3’ 144
Osteocalcin (NM_031368.4) F: 5’-CGGCCCTGAGTCTGACAAA -3’ R: 5’-GCCGGAGTCTGTTCACTACCTT -3’ 67
Ob-Ra (NM_001122899.1) F: 5’-ACACTGTTAATTTCACACCAGAG -3’ R: 5’-AGTCATTCAAACCATAGTTTAGG -3’ 236
Ob-Rb (NM_146146.2) F: 5’-ACACTGTTAATTTCACACCAGAG -3’ R: 5’-TGGATAAACCCTTGCTCTTCA -3’ 445
GAPDH (NR_003623.1) F: 5’-CCATCACCATCTTCCAGGAG -3’ R: 5’-GCATGGACTGTGGTCATGAG -3’ 303
Table 1: Oligonucleotide primers used in real-time RT-RCR and RTPCR (F: forward, R: reverse).
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