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| Figure 6: Changes in the mRNA expression of HIF-1 and SDF-1 in the BM of swines according to three time points following LAD intrumentation (baseline, 45 days, 90 days). Quantitative assessment of HIF-1, SDF-1, and GAPDH mRNA levels was performed by real-time RT-PCR. The relative quantitation value of the target is normalized to the endogenous control GAPDH (house-keeping) gene. Figure 6-a. HIF-1 was upregulated 45 days following the intervention and returned to baseline at the end of the study (90 days) (*P<0.001). Figure 6-b. SDF-1 mRNA expression was also initially increased (*P<0.001) and was significantly decreased at the end of the study (**P=0.03). |
