|
| Figure 6: Immunoblot analysis of LYPD6B. N-terminal V5 tagged isoforms of LYPD6B were expressed in OVCAR 3 cells. Cytosol and membrane protein fractions were extracted from transfectants and those proteins were analyzed by immunoblots. Lane 1, alpha-tubulin (MW=55 kDa) was used for cytosol specific marker. Lane 2, E-cadherin (MW=120 kDa) was used for membrane specific marker. Lane 3, anti-V5 tag antibody clearly detected V5-tag LYPD6B isofoms. Deduced MW of V5-tag LYPD6B_a, b and c were 28.1 kDa, 23.8k Da and 25.4 kDa, respectively. Lane 4, immunoblot result of ant-LYPD6B antibody. Deduced MW of LYPD6B_a, b and c were 23.3 kDa, 15.9 kDa and 20.6 kDa, respectively. |
