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| Figure 6: VZV ORF7 deletion virus in skin organ culture. A. Left panel is an image of ex vivo skin organ culture (SOC) maintained in Netwell inserts. Right panel is an image from the IVIS assay of the same SOC tissue infected with parental and ORF7 deletion (ORF7D) VZVLUC viruses. B. Ex vivo growth curve analysis of ORF7D VZVLUC in human fetal skin organ cultures (SOC). Skin tissues were inoculated with 5×103 PFU of either WT VZVLUC, ORF7D VZVLUC, or ORF7 rescue (ORF7R) VZVLUC viruses, in parallel. VZV replication was monitored daily by IVIS for one week as bioluminescence emitting from each skin culture was measured. Each line represents an average of the data from 3 different skin tissue samples, all infected with the same virus. |
