Figure 2: Endogenous iASPP-SV was present in many cancer cell lines. A. Expression of iASPP-SV was detected in cell lines using anti-iASPP antibody against C-terminus of iASPP (828), and transfected HEK 293 cells with pFLAG-iASPP-SV and pFLAG-iASPP (828) were used as positive control. Western blot using iASPP antibody was shown in left figure and FLAG antibody shown in right figure. B. Western blot (left figure) and Real-time PCR analysis (right figure) demonstrated that plasmids of antisense iASPP-SV suppressed endogenous iASPP-SV expression in MCF-7.
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