Figure 3: Amplification of the NAPPA signal with SNAP for MS identification of multiple protein-protein interaction. (left) Fluorescence analysis of SNAP Arrays; (middle, below) In vitro translation of a diverse set of target proteins using PURExpress system, improving by 20 fold the yield; (middle,above) MS Autoflex Bruker; (right) SpADS and Clustering solution for a specimen of 23 protein samples of raw data. Binning preprocessing function was performed for optimizing conclusive cluster analysis run on the ROI 1000/1200 spectra obtained by Autoflex Bruker MS (43).
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